Eradication of microorganisms from an infected root canal before obturation is a primary focus of endodontic treatment, as well as the best predictor for the long-term success of the endodontic therapy. The purpose of this in vitro laboratory study was to evaluate the efficacy of a new tapered and stripped Er:YAG laser tip using PIPS (Photon Induced Photoacoustic Streaming) in root canals infected with Enterococcus faecalis (ATCC4082). Methods: Twenty-four freshly extracted single-rooted human teeth were collected and inoculated with E. faecalis. After 4 weeks, the teeth were divided in four groups, two experimental, one positive, and one negative group. Laser treatment was performed for a period of 20 seconds with 6% sodium hypochlorite in groups 1 and 2, while PBS was used for group 3 and 4.
Furthermore, dentin debris was produced, and colony-forming units were determined. Results: The combinations of 20 seconds laser activated irrigation with Er:YAG Laser and 6% sodium hypochlorite showed 100% inhibition using PIPS of growth of E. faecalis compared to 50% inhibition with the combination of Er:YAG Laser and PBS. Conclusions: The PIPS technology is efficient tool in elimination of Enterococcus faecalis from infected root canals.
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